Title of Presentation or Performance
Bacteriophage Hercules11
Submission Type
Event
Expected Graduation Date
2017
Location
Center for Natural Sciences, Illinois Wesleyan University
Start Date
4-12-2014 9:00 AM
End Date
4-12-2014 10:00 AM
Disciplines
Biology
Abstract
The purpose of this project is to isolate and characterize one distinctive phage capable of infecting the bacteria, Mycobacterium smegmatis. Hercules11 was isolated from a soil sample collected outside of Munsell Hall on the Illinois Wesleyan University campus. The bacteriophage, or phage, was isolated via enrichment and further purified using streak assay techniques. Hercules11 was characterized as having a 2.0 mm diameter, transparent plaque morphology, lysogenic lifestyle, and unique DNA restriction patterns. North Carolina State University’s Genomic Sciences Laboratory sequenced the genome of Hercules11. After Auto-Annotation by DNA Master software, it was determined that Hercules11 had 90 genes comprised of 49,529 base pairs. A nucleotide BLAST revealed that Hercules11 is closely related to other phages including JHC117, Bxz2, and Jobu08, placing it in the A3 subcluster. In order to annotate and analyze the genome, the programs GeneMark, Glimmer, and DNA Master were used. Potential protein functions were compared to results from similar proteins using HHPred and BLASTP. Genome data will be submitted to the online Mycobacteriophage Database. Upon completion, the annotated genome will be submitted to GenBank, an online DNA database.
Bacteriophage Hercules11
Center for Natural Sciences, Illinois Wesleyan University
The purpose of this project is to isolate and characterize one distinctive phage capable of infecting the bacteria, Mycobacterium smegmatis. Hercules11 was isolated from a soil sample collected outside of Munsell Hall on the Illinois Wesleyan University campus. The bacteriophage, or phage, was isolated via enrichment and further purified using streak assay techniques. Hercules11 was characterized as having a 2.0 mm diameter, transparent plaque morphology, lysogenic lifestyle, and unique DNA restriction patterns. North Carolina State University’s Genomic Sciences Laboratory sequenced the genome of Hercules11. After Auto-Annotation by DNA Master software, it was determined that Hercules11 had 90 genes comprised of 49,529 base pairs. A nucleotide BLAST revealed that Hercules11 is closely related to other phages including JHC117, Bxz2, and Jobu08, placing it in the A3 subcluster. In order to annotate and analyze the genome, the programs GeneMark, Glimmer, and DNA Master were used. Potential protein functions were compared to results from similar proteins using HHPred and BLASTP. Genome data will be submitted to the online Mycobacteriophage Database. Upon completion, the annotated genome will be submitted to GenBank, an online DNA database.