Event Title

Bacteriophage Piro94

Graduation Year

2017

Location

Center for Natural Sciences, Illinois Wesleyan University

Start Date

12-4-2014 2:00 PM

End Date

12-4-2014 3:00 PM

Description

Soil samples from various locations across the Midwest were collecting during the fall of 2013. Through enrichment of the soil samples, novel phages were isolated, purified, and characterized. Using bacteriophage characteristics, such as plaque morphology, lifestyle and DNA restriction patterns, it was determined that Piro94 is unique from other phages isolated in the laboratory section. The bacteriophage, Piro94, was sent to the University of Pittsburgh for sequencing. After sequencing, it was determined that Piro94 is a member of subcluster A2. It has 52,647 base pairs and a 3’ overhang length of 10 base pairs. The 3’ overhang sequence was determined to be CGGTCGGTTA. The genome of Piro94 was subsequently annotated. Upon BLASTing the entire genome, it was discovered that Piro94 is most similar to the bacteriophages Echild, RedRock, Pukovnik, Turbido, and Trixie. The genome was annotated using a variety of software programs including GeneMark, Glimmer, Phamerator, HHPred, and DNA Master. There were a total of 92 genes and one tRNA for which the students determined the appropriate gene lengths and potential functions. All of the data regarding the bacteriophage, Piro94, will be submitted to Mycobacteriophage DataBase and the annotated genome will be submitted to the DNA database and GenBank.

This document is currently not available here.

Share

COinS
 
Apr 12th, 2:00 PM Apr 12th, 3:00 PM

Bacteriophage Piro94

Center for Natural Sciences, Illinois Wesleyan University

Soil samples from various locations across the Midwest were collecting during the fall of 2013. Through enrichment of the soil samples, novel phages were isolated, purified, and characterized. Using bacteriophage characteristics, such as plaque morphology, lifestyle and DNA restriction patterns, it was determined that Piro94 is unique from other phages isolated in the laboratory section. The bacteriophage, Piro94, was sent to the University of Pittsburgh for sequencing. After sequencing, it was determined that Piro94 is a member of subcluster A2. It has 52,647 base pairs and a 3’ overhang length of 10 base pairs. The 3’ overhang sequence was determined to be CGGTCGGTTA. The genome of Piro94 was subsequently annotated. Upon BLASTing the entire genome, it was discovered that Piro94 is most similar to the bacteriophages Echild, RedRock, Pukovnik, Turbido, and Trixie. The genome was annotated using a variety of software programs including GeneMark, Glimmer, Phamerator, HHPred, and DNA Master. There were a total of 92 genes and one tRNA for which the students determined the appropriate gene lengths and potential functions. All of the data regarding the bacteriophage, Piro94, will be submitted to Mycobacteriophage DataBase and the annotated genome will be submitted to the DNA database and GenBank.