Type of Submission

Event

Graduation Year

2014

Location

Center for Natural Sciences, Illinois Wesleyan University

Start Date

12-4-2014 9:00 AM

End Date

12-4-2014 10:00 AM

Disciplines

Chemistry

Abstract

Scanning Electrochemical Microscopy (SECM) utilizes electrode probes that can be positioned just abutting cells and can detect the presence of small quantities of chemicals known as neurotransmitters. In order to detect neurotransmitter release from PC12 cells, used as model neurons, the electrode must be positioned very close to the cell surface to detect the change in current that accompanies the oxidation/reduction of the neurotransmitters released. The peaks in current were analyzed in order to detect differences in release mechanisms, such as slow or fast-release. Furthermore, dual electrodes were made and characterized in order to monitor two different locations on a cell simultaneously. They can also be modified to observe two different electrochemical processes, such as amperometry and potentiometry.

Included in

Chemistry Commons

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Apr 12th, 9:00 AM Apr 12th, 10:00 AM

The Development and Characterization of Dual Electrodes for Use with Scanning Electrochemical Microscopy

Center for Natural Sciences, Illinois Wesleyan University

Scanning Electrochemical Microscopy (SECM) utilizes electrode probes that can be positioned just abutting cells and can detect the presence of small quantities of chemicals known as neurotransmitters. In order to detect neurotransmitter release from PC12 cells, used as model neurons, the electrode must be positioned very close to the cell surface to detect the change in current that accompanies the oxidation/reduction of the neurotransmitters released. The peaks in current were analyzed in order to detect differences in release mechanisms, such as slow or fast-release. Furthermore, dual electrodes were made and characterized in order to monitor two different locations on a cell simultaneously. They can also be modified to observe two different electrochemical processes, such as amperometry and potentiometry.

 

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