Event Title

Wrangling Warren: A Phage Discovery

Faculty Advisor

Richard Alvey

Faculty Advisor

David Bollivar

Graduation Year

2022

Location

Center for Natural Sciences, Illinois Wesleyan University

Start Date

13-4-2019 9:00 AM

End Date

13-4-2019 10:00 AM

Description

Bacteriophages are viruses that infect bacteria, found all over the world. Due to the number of bacteriophages, new phages are constantly being discovered and sequenced. Warren, a Microbacterium foliorum phage was discovered in a sample from Muncie, IL. It was isolated and had its DNA extracted at IWU. Using protocols such as TEM analysis and PCR, Warren seemed to have unique characteristics so it was sent to the University of Pittsburgh for genomic sequencing. The genomic sequence of Warren was used to place it in a new cluster of M. foliorum phages, the GA cluster, along with the previously sequenced Appa which was isolated in Pennsylvania. Annotation of Warren was completed using the program PECAAN, which uses GeneMark and Glimmer to identify the start sites of the genes and used phages from similar clusters as well as HHPred to identify gene function. Of the 66 genes annotated, 23 were given functions.

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Apr 13th, 9:00 AM Apr 13th, 10:00 AM

Wrangling Warren: A Phage Discovery

Center for Natural Sciences, Illinois Wesleyan University

Bacteriophages are viruses that infect bacteria, found all over the world. Due to the number of bacteriophages, new phages are constantly being discovered and sequenced. Warren, a Microbacterium foliorum phage was discovered in a sample from Muncie, IL. It was isolated and had its DNA extracted at IWU. Using protocols such as TEM analysis and PCR, Warren seemed to have unique characteristics so it was sent to the University of Pittsburgh for genomic sequencing. The genomic sequence of Warren was used to place it in a new cluster of M. foliorum phages, the GA cluster, along with the previously sequenced Appa which was isolated in Pennsylvania. Annotation of Warren was completed using the program PECAAN, which uses GeneMark and Glimmer to identify the start sites of the genes and used phages from similar clusters as well as HHPred to identify gene function. Of the 66 genes annotated, 23 were given functions.