Title of Presentation or Performance

Bacteriophage Zahn: Making Waves as a Singleton

Type of Submission

Poster

Type of Submission (Archival)

Event

Area of Study or Work

Biology

Faculty Advisor

Richard Alvey and David Bollivar

Expected Graduation Date

2022

Location

Center for Natural Sciences, Illinois Wesleyan University

Start Date

4-13-2019 9:00 AM

End Date

4-13-2019 10:00 AM

Disciplines

Education

Abstract

Bacteriophage research provides an opportunity to examine the evolution of a diverse group of biological entities. Using bacteriophages to treat bacterial infections is also a key component in biomedical research, as phages can be considered potential antibacterial agents due to their ability to infect and destroy bacteria. A bacteriophage from a water sample was isolated using bacterial host Rhodobacter capsulatus. Zahn was isolated in the summer of 2018 from West Lake in Davenport, Iowa. Using the bacterial host, the bacteriophage Zahn was isolated through the enrichment process. A lysate sample made after the purification of Zahn was used to create a TEM image, showing Zahn to have morphological uniqueness. After this discovery, a DNA preparation procedure to purify the phage DNA from the bacterial DNA was completed and it was sent to the Pittsburgh Bacteriophage Institute for sequencing. Zahn was determined to be a singleton, having no known close relatives, after acquiring the sequence. The genome of Zahn was then annotated using PECAAN. PECAAN was used to identify potential start sites and putative functions for Zahn’s genes. Once completed the annotated genome of Zahn will be submitted to GenBank to contribute to our knowledge of the evolution of bacteriophages.

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Apr 13th, 9:00 AM Apr 13th, 10:00 AM

Bacteriophage Zahn: Making Waves as a Singleton

Center for Natural Sciences, Illinois Wesleyan University

Bacteriophage research provides an opportunity to examine the evolution of a diverse group of biological entities. Using bacteriophages to treat bacterial infections is also a key component in biomedical research, as phages can be considered potential antibacterial agents due to their ability to infect and destroy bacteria. A bacteriophage from a water sample was isolated using bacterial host Rhodobacter capsulatus. Zahn was isolated in the summer of 2018 from West Lake in Davenport, Iowa. Using the bacterial host, the bacteriophage Zahn was isolated through the enrichment process. A lysate sample made after the purification of Zahn was used to create a TEM image, showing Zahn to have morphological uniqueness. After this discovery, a DNA preparation procedure to purify the phage DNA from the bacterial DNA was completed and it was sent to the Pittsburgh Bacteriophage Institute for sequencing. Zahn was determined to be a singleton, having no known close relatives, after acquiring the sequence. The genome of Zahn was then annotated using PECAAN. PECAAN was used to identify potential start sites and putative functions for Zahn’s genes. Once completed the annotated genome of Zahn will be submitted to GenBank to contribute to our knowledge of the evolution of bacteriophages.