Event Title

Separating the Effects of Water Viscosity and Temperature on the Clearance Rates of the Rotifer Brachionus plicatilis

Faculty Advisor

William Jaeckle

Graduation Year

2022

Location

Center for Natural Sciences

Start Date

4-4-2020 2:00 PM

End Date

4-4-2020 3:00 PM

Description

The viscosity and temperature of water affect swimming and feeding rates of small planktonic organisms. Water viscosity is influenced by temperature, therefore, change in temperature combines the separate influences of temperature and water viscosity. Owing to their small size, members of the phylum Rotifera are predicted to be strongly affected by the viscosity of their surroundings. We evaluated the influence of both viscosity and temperature on feeding rates of the marine rotifer Brachionus plicatilis. The clearance rates (volume of water cleared of particles) of rotifers exposed to 4.5 μm polystyrene beads (10,000/mL) were measured in three seawater treatments: (1) filtered (0.2 μm pore size) 15 ‰ sea water, 20 °C , (2) filtered 15 ‰ sea water, 10 °C, and (3) filtered 15 ‰ sea water, 20 °C where the kinematic viscosity was made equal to that of 10 °C seawater by the addition of the polysaccharide dextran. Following a 10-minute incubation, the beads present within each rotifer were counted. In each of three experiments, the clearance rates of B. plicatilis were significantly influenced by both temperature and viscosity (ANOVA, p < 0.001). Rotifers in the 20 °C treatment had the highest clearance rates, animals in the 10 °C treatment had the lowest clearance rates and individuals in the 20 °C (10 °C viscosity) treatment revealed intermediate clearance rates; within each experiment all treatments were significantly different (p < 0.05). The influence of water viscosity and temperature on feeding by Brachionus plicatilis averaged 57.7% and 42.3% respectively.

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Apr 4th, 2:00 PM Apr 4th, 3:00 PM

Separating the Effects of Water Viscosity and Temperature on the Clearance Rates of the Rotifer Brachionus plicatilis

Center for Natural Sciences

The viscosity and temperature of water affect swimming and feeding rates of small planktonic organisms. Water viscosity is influenced by temperature, therefore, change in temperature combines the separate influences of temperature and water viscosity. Owing to their small size, members of the phylum Rotifera are predicted to be strongly affected by the viscosity of their surroundings. We evaluated the influence of both viscosity and temperature on feeding rates of the marine rotifer Brachionus plicatilis. The clearance rates (volume of water cleared of particles) of rotifers exposed to 4.5 μm polystyrene beads (10,000/mL) were measured in three seawater treatments: (1) filtered (0.2 μm pore size) 15 ‰ sea water, 20 °C , (2) filtered 15 ‰ sea water, 10 °C, and (3) filtered 15 ‰ sea water, 20 °C where the kinematic viscosity was made equal to that of 10 °C seawater by the addition of the polysaccharide dextran. Following a 10-minute incubation, the beads present within each rotifer were counted. In each of three experiments, the clearance rates of B. plicatilis were significantly influenced by both temperature and viscosity (ANOVA, p < 0.001). Rotifers in the 20 °C treatment had the highest clearance rates, animals in the 10 °C treatment had the lowest clearance rates and individuals in the 20 °C (10 °C viscosity) treatment revealed intermediate clearance rates; within each experiment all treatments were significantly different (p < 0.05). The influence of water viscosity and temperature on feeding by Brachionus plicatilis averaged 57.7% and 42.3% respectively.