Title of Presentation

Illinois Wesleyan's Welcome Phage Annotation

Type of Submission

Pre-recorded Poster

Research Field

Biology

Faculty Advisor

Richard Alvey and David Bollivar

Graduation Year

2024

Start Date

10-4-2021 8:00 AM

End Date

11-4-2021 5:00 PM

Abstract

Viruses that infect bacteria are called bacteriophages and can be readily found in diverse environments. There are approximately 1031 bacteriophages on Earth. A search for new bacteriophages from the Illinois Wesleyan’s campus in Bloomington, Illinois led to the discovery of Welcome, a bacteriophage that welcomes you to learn more about these viruses. The plaques found were small and often had small bubbles in them. PCR was used to test for the relationship to known bacteriophages and lysogen testing was performed to determine if the bacteriophage could integrate into the host genome. TEM analysis placed this bacteriophage as a member of the Siphoviridae in the Caudovirales. The genome was sequenced at the University of Pittsburgh and analyzed using PECAAN, a bioinformatics software webpage. The PECAAN webpage compiled data from several analytical software analyses including HHPRED, PhagesDB, TmHmm, GeneMark, Glimmer, and NCBI Blast all of which were used when attempting to discern the function of a particular gene. The genome consists of 63,952 base pairs and approximately 122 genes. Welcome is one of only three other ED2 phages which have been analyzed.

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Apr 10th, 8:00 AM Apr 11th, 5:00 PM

Illinois Wesleyan's Welcome Phage Annotation

Viruses that infect bacteria are called bacteriophages and can be readily found in diverse environments. There are approximately 1031 bacteriophages on Earth. A search for new bacteriophages from the Illinois Wesleyan’s campus in Bloomington, Illinois led to the discovery of Welcome, a bacteriophage that welcomes you to learn more about these viruses. The plaques found were small and often had small bubbles in them. PCR was used to test for the relationship to known bacteriophages and lysogen testing was performed to determine if the bacteriophage could integrate into the host genome. TEM analysis placed this bacteriophage as a member of the Siphoviridae in the Caudovirales. The genome was sequenced at the University of Pittsburgh and analyzed using PECAAN, a bioinformatics software webpage. The PECAAN webpage compiled data from several analytical software analyses including HHPRED, PhagesDB, TmHmm, GeneMark, Glimmer, and NCBI Blast all of which were used when attempting to discern the function of a particular gene. The genome consists of 63,952 base pairs and approximately 122 genes. Welcome is one of only three other ED2 phages which have been analyzed.