Title of Presentation or Performance

The Isolation and Characterization of Rhodobacter capsulatus Phages Lkye and Aqua

Major

Biology

Type of Submission

Poster

Type of Submission (Archival)

Event

Area of Study or Work

Biology

Expected Graduation Date

2025

Location

CNS Atrium, Easel 21

Start Date

4-9-2022 8:30 AM

End Date

4-9-2022 9:45 AM

Abstract

Bacteriophages, commonly referred to as phages, are viruses that infect bacteria for the purpose of reproduction and the expansion of their species. The Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science Program, acronymed SEA-PHAGES Program, aims to increase the knowledge of the bacteriophage population through isolation and characterization of individual bacteriophages. The two phages studied were procured from water samples using the bacterial host known as Rhodobacter capsulatus. The isolated phages were identified as RcLkye and RcAqua, or simply Lkye and Aqua. Lkye and Aqua were discovered from enriched samples of water collected along Bloomington-Normal’s Sugar Creek in Illinois. The samples were collected five days apart and in separate locations. The phages were then purified via plaque assay and amplified for further analysis. It was discovered through Transmission Electron Microscopy (TEM) imaging that Lkye and Aqua were both siphoviridae phages, with similar capsid diameters and tail lengths. Based on comparisons of the sequenced genomes and TEM images of phages with confirmed clusters, it has been determined that Aqua and Lkye belong to the RcD cluster. The discovery and analysis of unique phages provide advancements in the field of microbiology and virology while potentially providing insight into combating viral diseases and infections.

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Apr 9th, 8:30 AM Apr 9th, 9:45 AM

The Isolation and Characterization of Rhodobacter capsulatus Phages Lkye and Aqua

CNS Atrium, Easel 21

Bacteriophages, commonly referred to as phages, are viruses that infect bacteria for the purpose of reproduction and the expansion of their species. The Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science Program, acronymed SEA-PHAGES Program, aims to increase the knowledge of the bacteriophage population through isolation and characterization of individual bacteriophages. The two phages studied were procured from water samples using the bacterial host known as Rhodobacter capsulatus. The isolated phages were identified as RcLkye and RcAqua, or simply Lkye and Aqua. Lkye and Aqua were discovered from enriched samples of water collected along Bloomington-Normal’s Sugar Creek in Illinois. The samples were collected five days apart and in separate locations. The phages were then purified via plaque assay and amplified for further analysis. It was discovered through Transmission Electron Microscopy (TEM) imaging that Lkye and Aqua were both siphoviridae phages, with similar capsid diameters and tail lengths. Based on comparisons of the sequenced genomes and TEM images of phages with confirmed clusters, it has been determined that Aqua and Lkye belong to the RcD cluster. The discovery and analysis of unique phages provide advancements in the field of microbiology and virology while potentially providing insight into combating viral diseases and infections.