Submission Type

Event

Expected Graduation Date

2011

Location

Center for Natural Sciences, Illinois Wesleyan University

Start Date

4-9-2011 9:00 AM

End Date

4-9-2011 10:00 AM

Disciplines

Chemistry

Abstract

The goal of the project is to develop the Scanning Electrochemical Microscopy (SECM) as a tool to study the biological effects of oxidative damage on rat pheochromocytoma cells (PCI2 cells). SECM is a useful tool for the analysis of biological samples because the ultramicroelectrode tip of the probe can detect the presence of electrochemically active compounds such as neurotransmitters, particularly dopamine and norepinephrine, while simultaneously characterizing the topography of the cell. The topography of the cell was determined by maintaining a constant distance between the tip of the electrode and the surface of the cell. In the collector mode, the potential of the SECM microelectrode can be set to detect dopamine release after stimulation. A spike in current indicated the release of neurotransmitters from the cell. Successful stimulation was observed on both undifferentiated and differentiated PC12 cells.

Included in

Chemistry Commons

Share

COinS
 
Apr 9th, 9:00 AM Apr 9th, 10:00 AM

Monitoring the Electrochemical Activity of Biological Samples Using Scanning Electrochemical Microscopy

Center for Natural Sciences, Illinois Wesleyan University

The goal of the project is to develop the Scanning Electrochemical Microscopy (SECM) as a tool to study the biological effects of oxidative damage on rat pheochromocytoma cells (PCI2 cells). SECM is a useful tool for the analysis of biological samples because the ultramicroelectrode tip of the probe can detect the presence of electrochemically active compounds such as neurotransmitters, particularly dopamine and norepinephrine, while simultaneously characterizing the topography of the cell. The topography of the cell was determined by maintaining a constant distance between the tip of the electrode and the surface of the cell. In the collector mode, the potential of the SECM microelectrode can be set to detect dopamine release after stimulation. A spike in current indicated the release of neurotransmitters from the cell. Successful stimulation was observed on both undifferentiated and differentiated PC12 cells.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.