Submission Type
Event
Expected Graduation Date
2014
Location
Center for Natural Sciences, Illinois Wesleyan University
Start Date
4-12-2014 9:00 AM
End Date
4-12-2014 10:00 AM
Disciplines
Chemistry
Abstract
Scanning Electrochemical Microscopy (SECM) utilizes electrode probes that can be positioned just abutting cells and can detect the presence of small quantities of chemicals known as neurotransmitters. In order to detect neurotransmitter release from PC12 cells, used as model neurons, the electrode must be positioned very close to the cell surface to detect the change in current that accompanies the oxidation/reduction of the neurotransmitters released. The peaks in current were analyzed in order to detect differences in release mechanisms, such as slow or fast-release. Furthermore, dual electrodes were made and characterized in order to monitor two different locations on a cell simultaneously. They can also be modified to observe two different electrochemical processes, such as amperometry and potentiometry.
Included in
The Development and Characterization of Dual Electrodes for Use with Scanning Electrochemical Microscopy
Center for Natural Sciences, Illinois Wesleyan University
Scanning Electrochemical Microscopy (SECM) utilizes electrode probes that can be positioned just abutting cells and can detect the presence of small quantities of chemicals known as neurotransmitters. In order to detect neurotransmitter release from PC12 cells, used as model neurons, the electrode must be positioned very close to the cell surface to detect the change in current that accompanies the oxidation/reduction of the neurotransmitters released. The peaks in current were analyzed in order to detect differences in release mechanisms, such as slow or fast-release. Furthermore, dual electrodes were made and characterized in order to monitor two different locations on a cell simultaneously. They can also be modified to observe two different electrochemical processes, such as amperometry and potentiometry.