The Isolation and Analysis of the Mycobacterium Phage Zonia

Submission Type

Event

Expected Graduation Date

2017

Location

Center for Natural Sciences, Illinois Wesleyan University

Start Date

4-12-2014 2:00 PM

End Date

4-12-2014 3:00 PM

Disciplines

Biology

Abstract

The mycobacterium phage, called Zonia, was isolated in Des Peres, Missouri. This phage was determined to be a virulent phage and part of the B1 subcluster. Zonia produced two distinct plaque morphologies ranging from 2-5mm; both were circular with a dark border. A high titer lysate with a concentration of 5.9*10^13 pfu/mL was used to isolate DNA. Through gel electrophoresis it was determined that the DNA was good quality and the concentration was 250 ng/µL. The DNA was sequenced at a facility in North Carolina and was determined to have 69,271 base pairs. The genome was annotated using GeneMark, Glimmer, HHpred, and DNA master and it was recognized to have 104 genes with various functions. Once the phage was annotated, similarities were found when compared to other phages in the B1 subcluster such as: Oline and YouGoGlencoco.

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Apr 12th, 2:00 PM Apr 12th, 3:00 PM

The Isolation and Analysis of the Mycobacterium Phage Zonia

Center for Natural Sciences, Illinois Wesleyan University

The mycobacterium phage, called Zonia, was isolated in Des Peres, Missouri. This phage was determined to be a virulent phage and part of the B1 subcluster. Zonia produced two distinct plaque morphologies ranging from 2-5mm; both were circular with a dark border. A high titer lysate with a concentration of 5.9*10^13 pfu/mL was used to isolate DNA. Through gel electrophoresis it was determined that the DNA was good quality and the concentration was 250 ng/µL. The DNA was sequenced at a facility in North Carolina and was determined to have 69,271 base pairs. The genome was annotated using GeneMark, Glimmer, HHpred, and DNA master and it was recognized to have 104 genes with various functions. Once the phage was annotated, similarities were found when compared to other phages in the B1 subcluster such as: Oline and YouGoGlencoco.