Regulation of Nmda through Fyn as a Result of Pulsed Radiofrequency in an Animal Model of Neuropathic Pain
Submission Type
Event
Faculty Advisor
Joseph Williams
Expected Graduation Date
2020
Location
Center for Natural Sciences, Illinois Wesleyan University
Start Date
4-21-2018 2:00 PM
End Date
4-21-2018 3:00 PM
Disciplines
Education
Abstract
For treatment of chronic pain, many specialists perform conventional radiofrequency (RF) ablation. Although resulting in a decrease in pain, side effects are correlated with the technique. To mitigate associated effects, some physicians prefer pulsed radiofrequency (PRF). PRF therapy may result in pain reduction while preventing side effects of RF, allowing for easier and more effective subsequent treatments. This work entails a proteomic analysis using an animal model to investigate chronic neuropathic pain and PRF treatment on an electro-biochemical level. Rat proteins from the ipsilateral dorsal spinal cord (SC) were extracted, separated, quantified, and identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Through an accepted vesiculation model and gene expression changes in Fyn, RACK1, and PTPRZ, we hypothesize that PRF alters the typical sequence of events on a molecular level associated with N-methyl-D-aspartate receptor (NMDAR) and the protein tyrosine kinase Fyn. Due to PRF treatment, NMDA receptor efficacy is decreased, as well as the potential for neuropathic pain signal propagation through a electro-biochemical pathway. Consequently, we conclude that PRF is an effective method of treatment for chronic pain management.
Regulation of Nmda through Fyn as a Result of Pulsed Radiofrequency in an Animal Model of Neuropathic Pain
Center for Natural Sciences, Illinois Wesleyan University
For treatment of chronic pain, many specialists perform conventional radiofrequency (RF) ablation. Although resulting in a decrease in pain, side effects are correlated with the technique. To mitigate associated effects, some physicians prefer pulsed radiofrequency (PRF). PRF therapy may result in pain reduction while preventing side effects of RF, allowing for easier and more effective subsequent treatments. This work entails a proteomic analysis using an animal model to investigate chronic neuropathic pain and PRF treatment on an electro-biochemical level. Rat proteins from the ipsilateral dorsal spinal cord (SC) were extracted, separated, quantified, and identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Through an accepted vesiculation model and gene expression changes in Fyn, RACK1, and PTPRZ, we hypothesize that PRF alters the typical sequence of events on a molecular level associated with N-methyl-D-aspartate receptor (NMDAR) and the protein tyrosine kinase Fyn. Due to PRF treatment, NMDA receptor efficacy is decreased, as well as the potential for neuropathic pain signal propagation through a electro-biochemical pathway. Consequently, we conclude that PRF is an effective method of treatment for chronic pain management.