Harboring Hartney: Making A Splash In Cluster B
Submission Type
Poster
Area of Study or Work
Biology
Faculty Advisor
Richard Alvey and David Bollivar
Expected Graduation Date
2022
Location
Center for Natural Sciences, Illinois Wesleyan University
Start Date
4-13-2019 2:00 PM
End Date
4-13-2019 3:00 PM
Disciplines
Education
Abstract
Bacteriophages, which are viruses, have the ability to infect numerous species of bacteria and are the most diverse biological entities on Earth, as roughly 10 31 exist. The discovery and sequencing of DNA from the newly discovered Rhodobacter capsulatus bacteriophage, Hartney, is used to further advance our understanding of the diversity of bacteriophages. Hartney was isolated from the Illinois River in Morris, Illinois. Hartney was obtained through enrichment and amplified. The genome was sequenced at NC State University and assembled at IWU to determine that Hartney belonged in Cluster B, which shows relation to Titan, Spartan, and Thunderbird. The tail and capsid sizes were determined through the use of Transmission Electron Microscopy (TEM) analysis at Indiana University. This analysis revealed that the tail size is 134 nanometers and the capsid diameter is 64 nanometers. The measurements indicate that the phage is a siphoviridae. Annotation of this phage was done using Genemark, Starterator, PECAAN, and Glimmer to determine the genes present. Once completed, these results will be submitted to GenBank.
Harboring Hartney: Making A Splash In Cluster B
Center for Natural Sciences, Illinois Wesleyan University
Bacteriophages, which are viruses, have the ability to infect numerous species of bacteria and are the most diverse biological entities on Earth, as roughly 10 31 exist. The discovery and sequencing of DNA from the newly discovered Rhodobacter capsulatus bacteriophage, Hartney, is used to further advance our understanding of the diversity of bacteriophages. Hartney was isolated from the Illinois River in Morris, Illinois. Hartney was obtained through enrichment and amplified. The genome was sequenced at NC State University and assembled at IWU to determine that Hartney belonged in Cluster B, which shows relation to Titan, Spartan, and Thunderbird. The tail and capsid sizes were determined through the use of Transmission Electron Microscopy (TEM) analysis at Indiana University. This analysis revealed that the tail size is 134 nanometers and the capsid diameter is 64 nanometers. The measurements indicate that the phage is a siphoviridae. Annotation of this phage was done using Genemark, Starterator, PECAAN, and Glimmer to determine the genes present. Once completed, these results will be submitted to GenBank.